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Figure 2 Comparison of short-term and long-term arecoline exposure on promoting locomotion hyperactivity. Arecoline induced a different pattern of hyperactivity behavior after short-term (30-min) and long-term (1-day) exposure in zebrafish larvae. Total average distance travelled per minute by 120 hpf zebrafish larvae during (A) light and (B) dark cycles after short-term (30-min) and long-term (1-day) arecoline exposure at different dosages were compared. The data are expressed as the median with 95% CI and significances were tested by the Mann-Whitney test. The statistical analyses were conducted between each treated group in every concentration (n = 48 for the control, 1-day exposure of 0.01 and 1 ppm, and 30-min exposure of 1 ppm arecoline groups; n = 47 for 1-day exposure of 0.001 ppm and 30-min exposure of 0.001 and 0.01 arecoline groups; n = 45 for 1-day exposure of 0.1 ppm and 30-min exposure of 0.1 ppm arecoline groups, **** p < 0.0001).