Figure 1

Figure 1

PPE31^Mm was required for the resistance of M. marinum to acid medium in vitro. (A) CRISPR interference has been performed for knockdown of ppe31^Mm. Expression of ppe31^Mm determined by RT-qPCR with or without induction of aTc. (B)M. marinum contain dCas9 and sgRNA targeting ppe31^Mm were exposed to 7H9 medium of pH4.5 with or without (control) induction of aTc. The bacterial survival was monitored by CFU counting at the indicated time. (C) WT, Δppe31^Mm, or comp-Δppe31^Mm were exposed to 7H9 medium of pH4.5 at 30°C. The bacterial survival was monitored by CFU counting for 9h. Data are shown as mean ± S.E.M. of three independent experiments. *p < 0.05, **p < 0.01.