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Figure Caption
Fig. 6 EC-specific Gpr182 is required for HSPC expansion in mouse FL. (A) The expression pattern of Gpr182 in mouse FL scRNA-seq data. (B) qPCR analysis of Gpr182 in ECs, LSK cells, and NC cells. Lyve1 and Hlf as positive markers for ECs and HSPCs, respectively. (C) The flowchart of ex-vivo siGpr182 knockdown and HSPC culture. (D) CFU-cell assays to detect the colony-forming ability of HSPCs following siRNA treatment.
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