ZFIN Image: Dhakal et al., 2021, FIGURE 12

Image

Figure Caption

FIGURE 12

Endothelial cell-depleted embryos are not hypoxic. (A) Quantitative (real-time) RT-PCR (qPCR) of prolyl-hydroxylase3 (phd3) transcripts shows increased phd3 levels in experimentally hypoxic embryos vs. normoxic controls (*p < 0.05; Mann-Whitney Wilcoxon, using ddCts). (B) qPCR shows no increase in phd3 levels in Met-treated, doubly-transgenic (cdh5:gal4; UAS:nfsB-mCherry) vs. DMSO-treated, doubly-transgenic controls (p = 0.89; Kruskal-Wallis with Conover post hoc, using ddCts; n = 4 per condition, where each biological replicate includes 3-4 pooled embryos).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol