Fig. 2

Fig. 2

Optimized mVenus fragment (VN155-I152L) increases the signal-to-noise ratio of TDP-43 complementation. a Representative microscope images demonstrating the fluorescence reconstitution in muscle tissue at 24 hpf using VN155-TDP-43 versus VN155-I152L-TDP-43 constructs. Scale bars represent 20 μm. b Quantitative comparison of signal-to-noise ratio using standard VN155 versus VN155-I152L fragments. Data was obtained using an unbiased plate reader. Data pooled and averaged from three independent replicates. c, d The background fluorescence (noise) was significantly reduced with the I152L mutation (c), while the maximum fluorescence intensity did not change (d). Data in c, d was obtained using an unbiased plate reader. Data pooled from three independent replicates. *P < 0.05; ****P < 0.0001; ^nsP > 0.05