ZFIN Image: Gordon et al., 2021, Fig. 4

Image

Figure Caption

Fig. 4 SFPQ directly binds to CLE-adjacent RNA sequences.

a Metaplot showing the distribution of predicted SFPQ-binding sites surrounding CLEs (red) and constitutive last exons of each CLE-containing gene (blue). In total, 200 bp of surrounding intron/CLE junction sequence (150 bp intron and 50 bp exon) were binned into 50 bp windows and the mean number of predicted motifs is shown ±SEM. b–d Top: location of SFPQ-binding motifs predicted using the MEME suite. Bottom: RT-qPCR quantitation showing the relative enrichment of SFPQ-interacting regions surrounding CLEs. RT-qPCR primer pairs were designed for each 100-bp sequence window demarcated by alternating gray and white areas. Abundance of SFPQ- or IgG(control)-crosslinked RNAs were normalized to input and the mean value from three replicates were shown ±SD. Source data are provided as a Source Data file.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.