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Fig. 5

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ZDB-IMAGE-210413-32
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Figures for Weinreb et al., 2021
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Fig. 5 Ddx41 regulates HSPC number via the cGAS-STING inflammatory pathway (A) Schematic of the cGAS-STING pathway knockdown experiments with HSPC quantification. (B) Flow cytometry plots of cd41:gfp+ HSPCs from ddx41 mutants injected with control morpholino (left), splice-blocking sting morpholino (middle), or translation-blocking (ATG) cgas morpholino (right) at 40 hpf. (C) Graph depicting the absolute number of cd41:gfp+ cells per embryo shown in (B). (D) Schematic of the STING knockdown and RNASEH1 overexpression experiment with HSPC quantification. (E) Flow cytometry plots of runx1:mcherry+ HSPCs from Tg(hsp70:M27RNASEH1-GFP)-negative (top left) and Tg(hsp70:M27RNASEH1-GFP)-positive (top right) uninjected ddx41 mutants versus Tg(hsp70:M27RNASEH1-GFP)-negative (bottom left) and Tg(hsp70:M27RNASEH1-GFP)-positive (bottom right) STING morpholino-injected ddx41 mutants at 40 hpf. (F) Graph depicting the number of runx1:mcherry+ HSPCs per embryo from (E). Graphs display means ± stds with p values calculated with a one-way ANOVA with Tukey’s multiple testing correction, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns, not significant (p > 0.05). N = 3–6 replicates per experiment.

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Reprinted from Developmental Cell, 56(5), Weinreb, J.T., Ghazale, N., Pradhan, K., Gupta, V., Potts, K.S., Tricomi, B., Daniels, N.J., Padgett, R.A., De Oliveira, S., Verma, A., Bowman, T.V., Excessive R-loops trigger an inflammatory cascade leading to increased HSPC production, 627-640.e5, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell