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Figure 8 Characterization of the molecular mechanisms underlying functions of Foxo1, ERK and p38 MAPKs in regulating cold resistance. (A) Samples used for RNA-seq. Zebrafish larvae at 96 hpf were exposed to 10 °C or maintained at 28 °C for 12 h in the presence of inhibitors for Foxo1 (AS: AS1842856, 0.5 μM), ERK (PD: PD0325901, 5 μM) or p38 MAPKs (SB: SB203580, 50 μM). DMSO is the vehicle control. (B) KEGG pathway enrichments of the DEGs between samples exposed to 10 °C in the presence of inhibitors (AS, upper panel; PD, lower panel) and the vehicle control (10 °C_DMSO). (C) Venn plots demonstrate the cold-responsive genes (CRGs) affected by the inhibitors. The genes up or down-regulated by cold stress (10 °C_DMSO vs. 96 hpf) were compared to those down-or up-regulated by the corresponding inhibitors (10 °C_AS/PD/SB vs. 10 °C_DMSO). (D) Heat map indicates the representative CRGs inhibited by AS1842856 and PD0325901. (E) Working model illustrating molecular mechanisms of Foxo1, ERK and p38 MAPKs in regulating cold resistance of zebrafish.