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Figure 6 Functions of ERK and p38 MAPKs in regulating cold resistance of zebrafish larvae. (A) Exposure to cold stress induced phosphorylation of ERK and p38 MAPKs. (B) Phosphorylation level of ERK and p38 MAPKs decreased during recovery at 28 °C. (C) PD0325901 treatment completely inhibited phosphorylation of ERK. (D) SB203580 treatment partially decreased phosphorylation of p38 MAPKs. The line charts below the Western blots indicate ratio of the phosphorylated ERK and p38 to the total protein. (E,F) Inhibiting the activity of ERK (E) and p38 (F) sensitized zebrafish larvae to cold stress. PD0325901 (5 μM) and SB203580 (50 μM) were added to the medium upon lethal cold exposure to inhibit the activity of ERK and p38, respectively.