|
Figure 4 Identification of potential molecular markers for cold-induced damage. (A) Venn analysis to identify genes exclusively affected by exposure to lethal cold stress. The 1134 genes representing the intersection among DEGs affected by lc, re and er (Figure 2E) were compared with the 2137 genes previously found to be upregulated by 24-h acclimation to a mild low temperature (18 °C) [17]. (B) Cluster dendrogram of the 959 genes exclusively related to lethal cold stress exposure. The genes were classified into 4 modules by WGCNA. (C) Heat map indicating eigengenes of the 4 modules. The number of genes in each module is shown on the right. The color scale indicates row z-score. (D) Enriched GO terms for the genes of module 1. The nodes represent GO terms and the edges indicate relationships between the nodes. Size of nodes is proportional to the number of genes associated with the term. The color scale indicates FDR (false discovery rate) of the enrichment analysis. (E) Western blots indicate changes in the level of phosphorylated histone H3 upon cold stress and during rewarming. Numbers below the blots are ratios of the phosphorylated histone H3 (p-H3) to total histone H3 (t-H3). (F) Network chart indicates hub genes of module 1. Names of the top 10 hub genes are highlighted. Size of the nodes is proportional to the maximal clique centrality.