IMAGE

Fig. 2

ID
ZDB-IMAGE-210408-8
Source
Figures for Zhang et al., 2021
Image
Figure Caption

Fig. 2 Tp1-positive BECs contribute to physiological hepatocyte homeostasis in a portion of zebrafish adults (A) Experimental scheme illustrating the stage of 4OHT treatment to triple transgenic line Tg(tp1:CreERT2; lfabp:loxP-STOP-loxP-DsRed; lfabp:Dendra2-NTR) from 5 dpf to 7 dpf and analysis at 1 and 2 years. (B) Live images showing the expression of Dendra2 and DsRed in the adult livers at 1 year. Co-immunostaining for Dendra2 and DsRed in the liver sections after DMSO and 4OHT treatment. (C) Fluorescence activating cell sorter (FACS) analysis showing the ratio of DsRed+ among Dendra2+ cells in DMSO- and 4OHT-treated livers. Quantification of the percentage of DsRed+ among Dendra2+ cells in DMSO- (n=3) and 4OHT-treated (no contribution, n=3; low contribution, n=4; high contribution, n=3) livers at 1 year. (D) Live images showing the expression of Dendra2 and DsRed in the adult livers at 2 years. Many clusters of DsRed+ cells among livers in 4OHT-treated groups. Co-immunostaining for Dendra2 and DsRed in the liver after DMSO and 4OHT treatment. (E) Fluorescence activating cell sorter (FACS) analysis showing the ratio of DsRed+ among Dendra2+ cells in DMSO- and 4OHT-treated livers. Quantification of the percentage of DsRed+ among Dendra2+ cells in DMSO- (n=5) and 4OHT-treated (no contribution, n=4; low contribution, n=5; high contribution, n=3) livers at 2 years. Numbers indicate the proportion of larvae exhibiting the expression shown. Asterisks indicate statistical significance: ∗p<0.05, ∗∗p<0.01, and p value was calculated by Student t tests. Scale bars: 100 μm. Data are represented as mean ±SEM.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience