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Figure 7

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ZDB-IMAGE-210327-68
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Figures for Ogawa et al., 2021
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Figure 7

Neuronal association between Tac1-immunoreactive processes and GnRH3 cell soma in the male and female zebrafish. Photomicrographs for immunofluorescence of GnRH3 (1st column, A−D), and Tac1 (2nd column, A’−D’) were merged (3rd column, A”−D”) to elucidate the possible neuronal association (arrows) between Tac1 and GnRH3 neurons. A representative GnRH3 cell soma accompanied by Tac1 processes was further examined by two-dimensional confocal scanning in XY, XZ, and YZ plane (4th column, A’”−D’”). Bar graphs in the bottom panel (E) show the percentage of GnRH3 cells accompanied by Tac1 processes in males (blue columns) and females (red columns) in the ventral nucleus of ventral telencephalic area (Vv) and olfactory-terminal nerve (OB-TN) region. Numbers in each columns indicate the number of GnRH3 cells accompanied by Tac1 processes and total GnRH3 numbers counted. In the Vv, more Tac1-immunoreactive processes (magenta) are in proximities of GnRH3 cells (green) in males (A−A’”) as compared to females (C−C’”), but there was no significant difference in the percentage of GnRH3 cells between males and females (E). In the olfactory bulb (OB), OB-GnRH3 neurons are accompanied by Tac1 processes in both males (B−B’”) and females (D−D’”), and there was no significant sex difference in the percentage of GnRH3 cells (E). ns, non-significant. Scale bars: 10 µm.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)