ZFIN Image: Giardoglou et al., 2021, Fig. 7.

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Figure Caption

Fig. 7.

PRKD2 association with TBX5a regulation. Multiple predicted transcription factor Tbx5a binding sites in the promoter region of zebrafish Prkd2 via the ConTraV3 web server (A) and the possible DNA binding motif (B). Brightfield image analysis and quantification of 72 hpf tbx5a-morpholino injected s411 siblings, tbx5a-morpholino injected wild-type and control wild-type embryos (C). tbx5-morpholino injected s411 mutant embryos exhibit an earlier more severe phenotype reminiscent to the tbx5 morphants and mutants. Scale bars: 100 μm. As measured via rt-qPCR, prkd2 expression levels in tbx5 morphants are reduced compared to uninjected siblings at 56 hpf, normalized both to act2b and elf1a as reference genes (D). n=6 independent replicates, 15 larvae per sample, Student's t-test (two-tailed distribution, paired), ** significantly different P-value <0.01, error bars +s.e.m.

Acknowledgments

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