Figure 1

Figure 1

Xenotransplantation of leukemia cells into SCID zebrafish. (A) Microscopic observation of K562 cells at 5 dpf zebrafish xenograft. (B) The 5 dpf zebrafish xenograft embryos were dissociated and labeled leukemia cells were analyzed manually using a hemocytometer. Error bars were calculated based on biological replicates in the experiment. Quantitative values were means ± SEM from 3–4 independent groups of 10 individuals at 3 dpf and 5 dpf. Control at 2 dpf represented the initial transplanted cells into the zebrafish from two separate experiments and showed a consistent amount of transplanted cells. Significance p values were determined as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.