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Fig. 2 Hoxb5b morphants have bifurcated foregut endoderm. (A and C) Control embryos; (B and D) Hoxb5b morphants; (E) Hoxb5a morphant. (A and B) In situ hybridization for pdx1 and (C–E) double in situ hybridization for foxa3 (blue) and insulin (magenta) for control and morphant embryos at 30 hpf. Anterior to the left (A and B) and to the top (C–E), results are from 3 independent experiments with a minimum of 45 embryos for Hoxb5b morphants, and from 2 independent experiments with a minimum of 20 embryos for Hoxb5a morphants.
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Reprinted from Developmental Biology, 471, Dalgin, G., Prince, V.E., Midline morphogenesis of zebrafish foregut endoderm is dependent on Hoxb5b, 1-9, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.