Fig. 6

Fig. 6 Fig. 6. Mapping NC cell lineages in zebrafish. A-C: Sox10-EGFP expression was in NC but not in taste buds. A: Merges EGFP and bright-field images of lateral views of Sox10-EGFP fish embryos at 7, 10, and 12-somite stages. Arrowheads point to heads and arrows point to truck regions. B: Photomicrographs of transverse sections of cranial regions of a 12-somite Sox10-EGFP fish embryo. Migrating NC cells were immunostained for Sox10 (magenta). C: Photomicrographs of a sagittal section of a taste bud in lower jaw of a 5.5 dpf fish embryo. D-E: Sox10-Cre/GFP-RFP labeled known NC-derived tissues but not taste buds. D: Whole mount images of gills (arrows) of Sox10-Cre/GFP-RFP adult zebrafish. E: Photomicrographs of sagittal sections of lower jaws at 15 dpf, 30 dpf and adult, and adult barbel of Sox10-Cre/GFP-RFP zebrafish. GFP and RFP signals were amplified by applying antibodies against GFP (green) and RFP (magenta). Calretinin (gray) indicates presence of taste buds. Scale bars: 200 ​μm in A and D; 50 ​μm in B; 20 ​μm in C and E (single-plane laser scanning confocal).