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Figure Caption
Figure 3 Generation of zebrafish abcc4−/− mutants with a clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system. The red color indicates the sgRNA target sequences for zebrafish abcc4 gene. (A) Diagram of the target site in the zebrafish abcc4 genome. (B) Abcc4 amino acids of wild-type (WT) and abcc4−/− mutants. (C) The predicted truncation of zebrafish Abcc4 protein. N and C indicate N terminal and C terminal, respectively. The red star symbol indicates a sgRNA targeting site of the abcc4 gene.
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Full text @ Int. J. Mol. Sci.