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Figure 2 Cellular functions of zebrafish Abcc4 in lead excretion. (A) The contents of monochlorobimane (MCB) in LLC-PK1 cells expressing zebrafish Abcc4 and transfected empty vectors (CTRL) with or without 25 μM MK571 were determined with dye accumulation assays after exposure to lead at the indicated concentrations for 24 h. (B) Survival rates of LLC-PK1 cells expressing zebrafish Abcc4 and CTRL with or without 25 μM MK571 were determined with 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assays after exposure to lead at the indicated concentrations for 24 h. (C) Contents of lead in LLC-PK1 cells expressing Abcc4 and CTRL after exposure to lead at the indicated concentrations for 12 h. (D) Contents of lead in LLC-PK1 cells expressing Abcc4 and CTRL after exposure to 50 μM lead at the indicated time points. Values are expressed as means ± SD (n = 3). Significant differences are indicated as * p < 0.05 and ** p < 0.01.