ZFIN Image: Shen et al., 2021, Figure 5.

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Figure Caption

Figure 5.

ZB transposon as a transgenic tool in mice. (A) ET constructs used in the mouse. The arrows represent TIRs; Myc, Myc minimal promoter; GFP, reporter (green fluorescent protein) gene; pA, Rabbit globin polyA. ZB transposase expression vector is mediated by SB transposon. SB, SB TIR; PGK2, human phosphoglycerate kinase 2 promoter; SV40En, SV40 enhancer. (B) The procedure used for the generation of a sperm mutant library, which is a double-transgenic mouse containing both a transposon cassette and a transposase expression cassette. Mutant libraries can produce pups with new integration sites after mating with female WT mice. (C) Fluorescence screening in three pups (nl5, nl9 and nl11) revealed the presence of GFP positivity at 10 days of age, while the founder (TnE2) was GFP negative. (D) Diagram of transposon insertions in the mouse genome. The red stars represent GFP-positive insertions, the blue stars represent GFP-negative insertions and the hollow blue star represents the founder (TnE2). (E) GFP expression patterns of nl5 in multiple tissues and organs at 5 days of age.

Acknowledgments

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