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Figure 1 Impact of daptomycin resistance on in vivo virulence and host immune responses. (A) Survival of the clinical DAP-R isolate A8817 and its parental DAP-S strain A8819 over 3 h in human blood (n = 4; * p < 0.05, Mann–Whitney test). (B) Survival of zebrafish following bloodstream infection with live S. aureus isolates (n = 30 embryos, three biological replicates; P value is a comparison of A8819 and A8817 by log-rank test). (C) Survival of the clinical DAP-R isolate A8817 and its parental DAP-S strain A8819 over 2 h in the presence of 20 μg/mL and 40 μg/mL hNP-1 (n = 3; for A8817 versus A8819, * p < 0.05 and ** p < 0.01 at indicated time points for same dosages, Student’s t-tests). Error bars represent the mean ± SEM. hNP-1: human neutrophil peptide 1.