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Fig. 4 Cl− uptake rates and whole body Cl− content of ca17a+/+, ca17a+/−, and ca17a−/− larvae (A and B), as well as sham Ca17a morpholino-treated larvae (C and D), and the effect of ethoxzolamide on Cl− uptake (E) in zebrafish (Danio rerio) larvae. There was a significant effect of genotype on Cl− uptake in 4- and 9-dpf larvae (A) (ANOVA; 4 dpf: F = 55.1, P < 0.01, n = 16 for ca17a+/+ and ca17a+/−, n = 12 for ca17a−/−; 9 dpf: F = 21.5, P < 0.01, n = 13 for ca17a+/+, n = 12 for ca17a+/−, n = 11 for ca17a−/−) but not Cl− content in 4 dpf (B) (ANOVA; F = 1.1, P = 0.36, n = 7 for ca17a+/+ and ca17a+/−, n = 6 for ca17a−/−) or 9 dpf (ANOVA; F = 1.9, P = 0.18, n = 8 for ca17a+/+ and ca17a+/−, n = 5 for ca17a−/−) larvae. There was an effect of Ca17a knockdown on Cl− uptake in 4-dpf zebrafish (C) (Welch’s two-sample t test; t = 3.5, P < 0.01, n = 6), but not Cl− content (D) (Welch’s two-sample t test; t = −0.8, P = 0.5, n = 6). There was an effect of ethoxzolamide on Cl− uptake in 4-dpf zebrafish (E) [one-way nonparametric ANOVA (Kruskal–Wallis); χ2 = 12.1, P < 0.01, n = 6]. Values with different letters are significantly different (P < 0.05) from one another. Data are presented as means ± SE. dpf, days postfertilization.