ZFIN Image: DeMarco et al., 2021, Figure 2

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Figure Caption

Figure 2

hspGGFF23C transgenic labels TL neurons that project to SM. (A,B) Low-resolution confocal images of 7 dpf triple transgenic Tg(hspGGFF23C,uas:egfp,isl2b:tagRFP) larva from side- and dorsal-view. (C) Higher magnification maximum projection images of TL and one tectal lobe of larva in (A,B). Merged fluorescence channel shown in (C), hspGGFF23C-driven EGFP channel shown in (C′). Note presence of EGFP-positive cells in TL and neurite plexus formed in tectal neuropil and lack of labeling in SPV layer of tectum. (D) Single confocal image through tectal neuropil. Note discrete EGFP labeling in the superficial SM layer of tectal neuropil. (E) Rotated side-view of image volume in (D), orientation orthogonal to the neuropil layers. (F) Fluorescence intensity profile measurement along line indicated by box in (E). Note peak in EGFP signal in the superficial SM layer. Scale bar: 300 μm in (A,B), 80 μm in (C), and 60 μm in (D,E).

Acknowledgments

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