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Figure 2—figure supplement 3. (A) Western blot of SATB2 showing retained expression in long term in vitro cultures derived from MCR:SATB2 tumors. Actin loading control is shown. (B) Overall survival and (C) histology of irradiated Casper recipients transplanted with MCR:EGFP Zmel1 (n = 31) or MCR:SATB2 45–3 (n = 31) zebrafish long term in vitro cultures. (D) Weekly prospective imaging of primary MCR:SATB2 individual tumor donor allograft in irradiated Casper recipient. Arrows indicate distant metastasis from injection site. Related to Figure 2H.
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