ZFIN Image: Fazio et al., 2021, Figure 2

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Figure Caption

Figure 2—figure supplement 1. SATB2 overexpression induces EMT, increased migration and invadopodia formation in zebrafish melanoma.

(A) Scratch assays on cultured MCR tumors show increased migration in MCR:SATB2 tumors. (B) Confocal analysis of immunohistochemistry of cultured MCR:SATB2 tumor shows co-localization of Cortactin (green) and F-actin (phalloidin, red), suggesting the formation of invadopodia. DAPI nuclear stain is shown in blue. (C) MCR:SATB2 (45–3) cells show increased Oregon green 488-conjungated gelatin degradation 24–25 hr post-seeding. Scale bar is 50 μm. (D) Percentage of zebrafish melanoma cells (n) with degraded 488-conjungated gelatin 24 hr post-seeding, observed in MCR (CK5, n = 93), MCR:SATB2 (45–3, n = 126), and MCR:SATB2 (63–4, n = 311) cell lines. (E) Immunofluorescence showing colocalization of F-actin and Cortactin in SKMEL2 overexpressing SATB2 in the presence of doxycycline (48 hr induction).

Acknowledgments

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