ZFIN Image: Fazio et al., 2021, Figure 1

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Figure Caption

Figure 1—figure supplement 3. SATB2 is not sufficient and unlikley necessary for melanoma initiation in zebrafish.

(A) Kaplan-Meier survival curves showing that MCR:SATB2 acceleration is dependent on BRAF^V600E and p53^-/-. Plotted: MCR:EGFP (n = 44) and MCR:SATB2 (n = 98) into Tg(mitfa:BRAF^V600E); p53^-/-; mitfa^-/-, compared to MCR:SATB2 into BRAF^-/-; p53^-/- (n = 44) and MCR:SATB2 into BRAF^+/+; p53^+/+ (n = 49). Log-rank (Mantel-Cox) test p values are shown. (B) T7E1 mutagenesis assay at the CRISPR target site in the satb2 gene. The assay was performed on genomic DNA from 2 dpf embryos injected at the one-cell stage with Cas9 mRNA and either a control gRNA (left) or a gRNA against satb2 (right). Cleavage bands (arrowheads, expected sizes 167 and 303 bp) indicate efficient mutagenesis at the target site. (C) Kaplan-Meier survival curves for p53 CRISPR (n-46), satb2 CRISPR-1 (n = 74), satb2 CRISPR-2 (n = 76) injected into [Tg(mitfa:BRAF^V600E); p53^-/-; mitfa^-/-] suggest satb2 to likely be dispensable for melanoma initiation. Log-rank (Mantel-Cox) test, NS; not significant, ****p<0.0001. (D) Kaplan-Meier survival curves for MCR:SATB2 (n = 98), MCR:SATB1 (n = 107), and MCR:EGFP (n = 44) into Tg(mitfa:BRAF^V600E); p53^-/-; mitfa^-/- showing that unlike SATB2, closely related gene SATB1 does not accelerate melanoma onset. Log-rank (Mantel-Cox) test, NS; not significant, ****p<0.0001.

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