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Figure 1—figure supplement 2. SATB2 overexpression leads to invasive melanoma with organ involvement.

(A) Western blot and (B) immunofluorescence validation of human SATB2 protein expression in MCR:SATB2 zebrafish primary tumors. (C) Cross-section through zebrafish mounted in a paraffin block. Compared to the MCR:EGFP-injected zebrafish which has a pigmented tumor with confined borders, in the MCR:SATB2-injected zebrafish dispersed pigmented tumor cells can be observed throughout the body. MCR:SATB2-injected zebrafish have increase organ involvement, as shown by pigmented melanoma cells (white arrow) in the (D) intestine, and (E) pancreas. (F) Histological aspect of MCR:SATB2 tumors illustrating spindle morphology of melanoma cells.

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