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Fig. 4 Evidence of the presence of a repressive element within a 22 bp cLEN sequence. (A) Transgene constructs examined for reporter gene expression. Endocardial expression results are summarized on the right. EC, endocardial cell. Uninj. and Reg. correspond to uninjured and 3 dpa regenerating hearts, respectively. (B) Images of sections of transgenic fish carrying cLEN deletion transgenes. Top, uninjured hearts. Middle, the remote zone of 3 dpa hearts. Bottom, the border zone of 3 dpa hearts. The arrows indicate endocardial EGFP expression. (C) Quantification of EGFP+ area per 100 µm2 cardiac tissue in uninjured hearts. (D) Quantification of EGFP+ area per 100 µm2 cardiac tissue in the border zone of 3 dpa hearts. (E) Quantification of EGFP+ area per 100 µm2 cardiac tissue in the remote zone of 3 dpa hearts. The data are presented as the mean±s.d. Numbers of animals are shown in Table S3. *P<0.05; **P<0.01; ***P<0.001; N.S., not significant. One-way ANOVA with Tukey's post-hoc test. Scale bar: 100 µm in B.