Fig. 5

Fig. 5 A subpopulation of Tbx5a-positive cells fails to migrate from the pLPM to the pectoral fin bud in Cdx-deficient embryos. (A–L) Maximum Intensity Projections (MIPs) of live-imaged Tg(tbx5a:eGFP), Tg(h2afx:h2afv-mCherry)mw3 double transgenic embryos, oriented dorsally. The fin field cells that completed migration into the fin bud region are indicated by the arrowheads in Tg(tbx5a:eGFP) (A–D) and Tg(h2afx:h2afv-mCherry)mw3 (E–H) embryos at 24 hpf. Cells that were backtracked beginning at 24 hpf are indicated by the colored dots (E–H). The tracks for the selected cells are indicated by colored lines in Tg(h2afx:h2afv-mCherry)mw3 embryos at 18 hpf (I–L). The fin bud region at 24 hpf is shown for wt (white arrowheads, A, E), Cdx-deficient (red arrowheads, B, F), DMSO-treated (white arrowheads, C, G), and Talarozole-treated (yellow arrowhead, D, H). The somite level of the anterior and posterior fin bud region is indicated (either s1 and s4 or s1 and s6). The somite level that Tbx5a-positive cells of the fin bud originated from at 18 hpf are quantified for DMSO and Talarozole-treated embryos (M) or for wt and Cdx-deficient embryos (N). The number of cells tracked/the number of embryos used is indicated per condition as a fraction at the bottom of tables M–N. (O) Quantification of the length of the fin bud region at 28 hpf for DMSO (yellow), Talarozole (blue), Wt (navy blue) and Cdx-deficient (brown). Statistical significance was determined using the student t-test (**, p < 0.01) Non-significant differences are indicated as NS. Scale bar represents 100 μm.