Fig. 3

Fig. 3 Increased RA or Cdx4 results in mis-patterning in the anterior LPM. (A–L) In situ hybridization at 14 hpf for aLPM markers scl-1 (A–D), tbx5a (E–H), and hand2 (I–L) in dorsal view. The AP length of scl-1 expression in HS controls (black bracket, A) is truncated in length in Hsp70.cdx4 (B). Compared to the expression length of scl-1 in DMSO controls (C) scl-1 expression is also truncated in Talarozole-treated embryos (red arrowhead, D). The anterior boundary of tbx5a expression in the aLPM relative to expression in the eye (black bracket, E) is shifted rostrally in Hsp70.cdx4 (red arrowhead, E), compared to HS controls (E). Expression of tbx5a in the aLPM of Talarozole-treated embryos (red arrowhead, H) is also shifted rostrally, compared to DMSO-treated controls (G). The anterior boundary of hand2 in the aLPM is unaffected between HS controls and Hsp.70.cdx4 (I, J). Expression of hand2 shifts rostrally in Talarozole-treated fish (red arrowhead, L) compared to DMSO-treated controls (K). Expression of tbx5a in the pLPM at 18 hpf is shifted rostrally in Hsp70.cdx4 embryos (red arrowhead, N) compared to HS controls (white arrowheads, M). Expression of tbx5a is also shifted rostrally in Talarozole-treated fish (red arrowhead, P) compared to DMSO-treated controls (white arrowheads, O). (Q) Quantification of the AP length of scl-1 in the aLPM normalized to the trunk width (brackets in A). (R) Quantification of the length between tbx5a expression in the aLPM and eye (bracket, E), normalized to the width of the trunk. Statistical significance was determined using the student t-test (*, p < 0.05, ***, p < 0.001, ****p < 0.0001). Scale bars represent 100 μm.