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Fig. 6 PDIM’s methyl-branched mycocerosic acids promote mobility and spreading. (A) Phthiocerol di-fatty acid (PDIF) and azido-DIF synthesis. (B) Fluorescent recovery curves after photobleaching of DIM-488 or DIF-488 labeled M. marinum, lines represent the average signal from n = 9–10 cells. (C) Mean fraction mobile which is the plateau following fitting of data generated in B to a non-linear regression with a one-phase association. Two-tailed, unpaired t test. Images of M. marinum expressing a blue-fluorescent protein recoated with (D) DIM-488 or (E) DIF-488 at 2 hpi into the HBV of wildtype fish, arrows indicate spread signal, scale bar = 5 μm. (F) Mean percent DIM-488 or DIF-488 in macrophage or epithelial cells no longer localized with bacteria following HBV infection with ~100 M. marinum. Two-tailed Mann Whitney test for 2 hpi and two-tailed, unpaired t test for 24 hpi. (B), (C), and (F) representative of three separate experiments.