ZFIN Image: Noschka et al., 2021, Figure 4

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Figure Caption

Figure 4

In silico design of Angie1 from Angiogenin. (A) Sequence of Angiogenin precursor (1–24: signal peptide; 25–147: Angiogenin). The cytolytic active site was determined by AMPA (64–80aa) and is highlighted in green. (B) Helical wheel projection of the cytolytic region (left panel) demonstrating the polarity of the amino acids. The right projection shows the modifications of the cytolytic region yielding Angie1. (C) Mass spectrometry analysis of Angie1. Angie1 was analyzed by LC-ESI-MSMS (Orbitrap Elite system). The spectrum shows the [M+2H]²⁺, [M+3H]³⁺, and [M+4H]⁴⁺ multicharged signals with the monoisotopic m/z value.

Acknowledgments

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