Fig. 3

Fig. 3 Fig. 3. Lamellipodia-like protrusions interact with membranes of skin and pLLP cells. (A,B) Basal layer epidermal cells were mosaically labeled by injection of krt4:mCherry-CAAX plasmid in a TgBAC(cxcr4b:LifeAct-Citrine) embryo and imaged between 28 and 32 hpf (Movie 4). Panels A and B show top-down view of the pLLP. Live movies were processed in Imaris to generate a 3D surface rendering for each channel. Gray color in A′ and B′ indicates contact between the protrusions and the membranes (magenta). Note that pLLP cells make direct contact with the overlying skin cells. (C–E) Donor cells derived from TgBAC(cxcr4b:LifeAct-Citrine) and Tg (sox10:memRFP) (magenta) were transplanted into non-transgenic embryos and mounted for live imaging between 30 and 36 hpf (Movie 5). Panels C and D show lateral views. Panels C′ and D′ show digitally rotated top-down view from C and D (horizontal dashed line). C″ and D″ panels show a digitally rotated coronal section through the pLLP in C and D (vertical dashed lines). Surface-to-surface contact shown in gray. Note the close proximity of the green protrusion to the magenta membrane (boxes in C′, C″, D′, and D″). Panel E shows contact of a cell, incorporated into rosette, with another cell in front (box). Panel E′ shows surface-to-surface contact rendering of cells in the region. Panel E″ show a digitally rotated coronal section through the dashed line in E. Note the close proximity of the protrusion to the membrane of a neighboring cell (arrow). Scale bar ​= ​10 ​μm.