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Figure 5—figure supplement 2. 4T1 cells and extracellular vesicles (EVs) express CD146/MCAM long isoform.

(a) Western blots on 4T1 cell and EVs extracts using antibodies selectively recognizing CD146/MCAM short (left) and long (right) isoforms. (b) Anti-CD146 Elisa on EVs isolated from shControl, shRalA, and shRalB cells. Each dot represents one replicate. Horizontal line represents the median. (c) Representative confocal images of 4T1 cells showing endogenous expression of CD146 and CD63 by immunofluorescence. Scale bars: 10 μm; zoom: 2 μm.

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