Figure 3—figure supplement 1.

Figure 3—figure supplement 1.

(a) Graph showing the percentage of cells expressing caspase 3 assessed by immunofluorescence on breast primary tumors. Each dot represents one mouse (eight mice taken from two independent experiments; One-Way Anova followed by Bonferroni's Multiple Comparison Test). (b) Graph showing the caspase3/7 expression levels on protein extracts from breast primary tumors. Each dot represents (12–19 mice from two independent experiments; Kruskal-Wallis test followed by Dunn's Multiple Comparison Test) (c) Graph showing the proliferation of 4T1 shControl, shRalA, and shRalB cells over time in an in vitro assay Two-way ANOVA followed by Bonferroni's Multiple Comparison Test (three independent experiments with n = 5). (d) Graph showing the cycle analysis of 4T1 shControl, shRalA, and shRalB cells. (e) Graph showing the effect of high and low doses of 4T1 extracellular vesicles (EVs) on permeabilization of a HUVEC monolayer in vitro. The graph represents the normalized amount of fluorescent dextran that crossed the endothelial barrier. Each dot represents one experiment (three independent experiments; One-Way Anova permutation test followed with fdr multicomparison test).