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Figure 2—figure supplement 1. PLD1 and PLD2 in 4T1 cells.

(a) PLD1 and PLD2 expression in 4T1 shControl cells quantified by qRT-PCR and normalized by gapdh expression. (b) Representative confocal images showing PLD1-GFP and PLD2-GFP subcellular localization in 4T1 cells stained with lysotracker. Scale bars: 10 μm (c) Representative confocal images of PLD2-GFP localization in shControl, shRalA, and shRalB cells. Scale bar: 10 μm (d) PA/PC ratio of species known to be targeted by PLD1 identified in extracellular vesicles (EVs) isolated from shControl, shRalA, and shRalB cells. Each dot represents one experiment; three independent experiments.

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