ZFIN Image: Ghoroghi et al., 2021, Figure 1

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Figure Caption

Figure 1—figure supplement 2. Electron microscopy analysis of endosomes in the absence of RalA or RalB (<bold>a-b</bold>).

Graph showing the number of multi-vesicular body (MVB) (a) or endolysosomes (b) per cytoplasm surface in electron microscopy analysis performed by chemical fixation or high-pressure freezing on 4T1 shControl, shRalA, and shRalB cells. Each dot represents one field of view. Kruskal-Wallis test followed by Dunn's Multiple Comparison Test. (c) FACS analysis of 4T1 shControl, shRalA, and shRalB cells stained with Lysotracker. Each dot represents one experiment (five independent experiments; Kruskal-Wallis test followed by Dunn's Multiple Comparison Test). (d) Representative electron micrographs of MVBs in 4T1 shControl, shRalA, and shRalB cells after chemical fixation or high-pressure freezing. Scale bar: 200 nm. (e–f) Number of ILV per MVB surface (e) and MVB diameter (f) measured in electron microscopy analysis performed by chemical fixation or high-pressure freezing on 4T1 shControl, shRalA, and shRalB cells. Each dot represents one MVB; Kruskal-Wallis test followed by Dunn's Multiple Comparison Test.

Acknowledgments

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