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Figure 1—figure supplement 1.

ID: ZDB-IMAGE-210128-61
Publication: Ghoroghi et al., 2021 - Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes
All Figures: Figures for Ghoroghi et al., 2021

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Figure Caption

Figure 1—figure supplement 1. Ral knockdown efficiency and impact on EV secretion.

(a) Representative western blots showing tubulin, RalA (left) and RalB (right) expressions in 4T1 shControl, shRalA, and shRalB cells. (b) Graph showing the average diameter of the extracellular vesicles (EVs) isolated from 4T1 shControl, shRalA, and shRalB cells measured by nanoparticle-tracking analysis. Each dot represents one experiment (12 independent experiments; One-Way Anova followed by Bonferroni's Multiple Comparison Test). (c) Nanoparticle-tracking analysis of EVs isolated by ultracentrifugation (100,000 g pellet) from the supernatant of various breast cancer cell lines treated with the Ral inhibitor RBC8. Each dot represents one experiment (four independent experiments, Mann Whitney test, p value indicated on the graph).

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