ZFIN Image: Zhou et al., 2021, Figure 6

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Figure Caption

Figure 6 Analysis of integration site of IFN1-expressing cassette in transgenic loaches. (A) Genomic DNA of grass carp IFN1 cDNA-transgenic loaches was digested with EcoRV and subjected to Southern blotting with digoxigenin-labelled cDNA probes and antidigoxin antibodies. (B) Schematic diagram of the transposon-mediated IFN1-expressing cassette integrated in the genome, showing primer pairs krt8-F1/IFN1-R5 for Southern blots. (C) The electrophoresis of genome walking. AD5 and AD1 are merging primers for the genome of loach. R1, R2, R3 and L1, L2, L3 are primers designed on integrated IFN1-expressing cassette of the plasmid pT2-krt8-IFN1.

Acknowledgments

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