ZFIN Image: Guo et al., 2021, Figure 3

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Figure Caption

Figure 3

Effects of lhx4 knockdown via vivo-MO in the eyes on the other retinal neuronal differentiation. All figures are horizontal sections along the temporal-nasal axis (T-N). (A–D”) Immunofluorescence staining with other retinal neural markers, PKCα (at 84 hpf), Zpr1(at 84 hpf), Rhodopsin (at 84 hpf), and Zn8 (at 72 hpf) in WT, vCT, and 4vMO retinas. Blue, DAPI staining of the nuclei. Scale bar = 20 μm. (a–d) Statistical analysis of PKCα⁺, Zpr1⁺, Rhodopsin⁺, and Zn8⁺ cells in WT, vCT, and 4vMO retinas. ns, P > 0.05; ****, P < 0.0001; vCT vs. WT; 4vMO vs. vCT. Results are presented as the mean ± SEM (n ≥ 10).

Acknowledgments

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