ZFIN Image: Everson et al., 2020, Fig. 3

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Figure Caption

Fig. 3

Mutations in shha sensitize embryos to multifactorial interactions between ethanol and PBO. (A) Wild‐type (+/+) or heterozygous (+/‐) embryos for shha (tq252) were exposed to subthreshold doses of PBO (3.125 μM), ethanol (0.5%), or the combination of both chemicals (3.125 μM PBO + 0.5% ethanol). Percent malformations (mild, moderate, and severe) are shown. Incidence of malformations was compared between genotypes for each treatment group using Fisher’s exact test with Bonferroni correction for multiple comparisons. (B) Quantification of inter‐trabecular widths. Mean width ± SEM is shown. Measurements were normalized to control and compared using one‐way ANOVA with Tukey’s multiple comparisons test. Different letters indicate statistically significant differences between the groups. See supplemental data for the specific multiple comparisons p‐values. N ≥ 14 embryos per genotype per treatment.

Figure Data

Phenotype details

Acknowledgments

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