On the left the phylogenetic tree depicts the relationship between the Danio species; the asterisk denotes a node with lower bootstrap support. The left column shows the patterns of the different species. In the middle column patterns of wild-type hybrids with D. rerio are shown (see also Supplementary Fig. 1). In the right column patterns of hybrids that carry a mutant Kcnj13 allele from D. rerio are shown. Pattern defects are obvious in three cases: hybrids with D. aesculapii (n > 60, magenta), with D. tinwini (n = 12, yellow) and D. choprae (n = 40, cyan). In the other six cases the patterns in hemizygous hybrids do not differ from the striped patterns of wild-type hybrids (D. rerio Kcnj13 k.o./D. kyathit, n = 32; D. rerio Kcnj13 k.o./D. nigrofasciatus, n = 16, D. rerio Kcnj13 k.o./D. albolineatus, n = 4; D. rerio Kcnj13 k.o./D. erythromicron, n = 38; D. rerio Kcnj13 k.o./D. margaritatus, n = 12; and D. rerio Kcnj13 k.o./D. dangila, n = 16). All pictures show representative examples of the corresponding species/hybrids/genotypes; for variability of the hybrid patterns see also Supplementary Fig. 3. Scale bars correspond to 1 mm.
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