ZFIN Image: Dalle Nogare et al., 2020, Figure 7.

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Figure Caption

Figure 7. Superficial and basal protrusion morphology and directionality after removal of the overlying skin and embedding in Matrigel.

(A) Superficial LifeAct-positive protrusions (green) and membrane (magenta) in skin-removed embryos embedded in 20 mg/mL Matrigel. (B) Quantification of superficial protrusion directionality in embryos embedded in Matrigel (C) Basal LifeAct-positive protrusions (green) and membrane (magenta) in skin-removed embryos embedded in 20 mg/mL Matrigel (D) Quantification of basal protrusion directionality in embryos embedded in Matrigel. (E) Box plots of average cell migration speed (in the direction of normal migration) for embryos with skin intact embedded in 1% agarose, skin removed embedded in 1% agarose, skin removed and regrown embedded in 1% agarose, skin intact in 20 mg/mL Matrigel, and skin removed embedded in 20 mg/mL Matrigel. Dots indicate individual cell speed in the direction of normal migration in at least three embryos. p Values are represent results from ANOVA with multilevel model. Panels (A, C) are processed Airyscan super-resolution images.

Acknowledgments

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