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Fig 3

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ZDB-IMAGE-201121-5
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Figures for Schindler et al., 2020
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Fig 3 Evaluation of wt1a expression <italic>in vivo</italic> and immunostaining of cryosections for the slit membrane protein nephrin in ADR-treated larvae.

2-PM in vivo imaging revealed no morphological changes or glomerular abnormalities in treated larvae (A). Immunostainings showed that ADR treatment neither reduces the expression of the pronephric transcription factor wt1a nor changes the expression pattern of nephrin (B). RT-PCR (C) and RT-qPCR (D) confirmed that nephrin mRNA expression was not altered in ADR-treated larvae at 9 dpf. Scale bars represent 10 μm.

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