ZFIN Image: Zhang et al., 2020, Figure 3

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Figure Caption

Figure 3 GFP expression after the injection of zebrafish enhancers.

Lateral views of zebrafish embryos with heart-specific GFP expression after injection with tol2 mRNA and tnni1b-ECR183 (A–C) or tnni1b-ECR183-d2 (D–F), scale bars = 100 µm. The average ratios of heart-specific GFP expression embryos to the total surviving embryos were 10/45 (22%) at 24 hpf, 15/41 (37%) at 48 hpf, and 15/39 (38%) at 72 hpf in the tnni1b-ECR183 group and 7/59 (12%) at 24 hpf, 18/48 (38%) at 48 hpf and 16/43 (37%) at 72 hpf in the tnni1b-ECR183-d2 group. (G) Rate of embryos with GFP expression in different tissues at 48 hpf after injection with tnni1b-ECR183. (H) Rate of embryos with heart-specific GFP expression from transient injections of tnni1b-ECR183 and each truncated enhancer with tol2 transposase at 48 hpf. A t-test was used for statistical analyses between the two groups (G). One-way ANOVA was used to test for homogeneity of variance, and Bonferroni’s test was used to correct for multiple comparisons (H). ** p < 0.01; *** p < 0.001; **** p < 0.0001, n = 3.

Acknowledgments

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