ZFIN Image: Diofano et al., 2020, Fig 5

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Figure Caption

Fig 5 Bag2 mediates genetic compensation in <italic>bag3</italic> knockout zebrafish.

(A) Schematic description of proteomic workflow. Acquired spectra were analyzed against the Uniprot zebrafish database using MaxQuant. (B) Detection levels of label free quantification (LFQ) intensity ratio between bag3^-/- and bag3^+/+ zebrafish samples of three orthologs of the Bag family protein, Bag1, Bag2 and Bag3. The values are shown as Log2 fold change ≥2 and ≤2. (C) Quantitative real-time PCR of bag3^-/- and bag3^+/+ zebrafish embryos at 72 hpf shows significant upregulation of bag2 mRNA levels, whereas bag1 transcript levels are not increased (N = 3, mean±S.D, One-way ANOVA followed by tukey's multiple comparison analysis **bag1 vs bag2 P = 0.0057, ** bag2 vs bag3 P = 0.0026). (D) Quantitative real-time PCR of MO-bag3- or MO-bag3 5bp mm-injected embryos shows no upregulation of bag2 mRNA levels in bag3 morphants at 72 hpf. (n = 3, mean±S.D, P = 0.1080 determined using two-tailed t-test).

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Acknowledgments

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