ZFIN Image: Knüfer et al., 2020, Figure 6

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Figure Caption

Figure 6 Perturbation of cadherin function does not disrupt zebrafish extraocular neuromuscular junction formation.

(A–H’’) Immunostained NMJs composed of presynaptic vesicles (SV2) and acetylcholine receptors (α-bungarotoxin) in wild-type (A–D’’) and cdh2ΔEC (E–H’’) larvae at superior rectus (SR), inferior rectus (IR), inferior oblique (IO) and superior oblique (SO) extraocular muscles at 4 dpf. Scale bars = 20 μm. All images are maximum intensity projections of sub-stacks taken from larger z-stacks. (I) Area of SV2+ signal and colocalisation coefficient of SV2 and α-bungarotoxin (J) of NMJs of each individual extraocular muscle. There was no significant difference in the area of SV2 (SR, p=0.9880; MR, p=0.9391; IR, p=0.9880; IO, p=0.9880; SO, p=0.9751; multiple t-tests, and Holm-Sidak test) or colocalisation of SV2 (SR, p=0.9288; MR, p=0.9288; IR, p=0.9212; IO, p=0.9288; SO, p=0.9534) and α-bungarotoxin for any muscle between wild types (n = 7 for MR, IO, SO; n = 6 for SR and IR; from two clutches) and isl1:cdh2ΔEC-mCherry (n = 7 for SR, MR, IR, IO; n = 6 for SO; from two clutches). Error bars are mean and SD.

Figure 6—figure supplement 1—source data 1.

Acknowledgments

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