Figure 4.

Figure 4.

Umbilical cord blood-derived hematopoietic stem and progenitor cells show engraftment, self-renewal and multilineage differentiation in GSS larvae. (A) Representative image of GSS larvae and casper control larvae transplanted with umbilical cord blood (UCB)-derived hematopoietic stem and progenitor cells (HSPC). Image shows near complete absence of HSPC in control larvae at 28 hours post-injection (hpi), whereas the HSPC continue to survive in the GSS larvae at 28 hpi and HSPC are seen until 72 hpi (N=20/genotype). Image specification: magnification =10x, numerical aperture = 0.3. White and black scale bars represent 100 μm and the yellow scale bar represents 50 μm. (B) Heatmap from RNA sequencing analysis of transplanted HSPC shows the increased expression level of self-renewal specific genes in HSPC transplanted into GSS larvae. Control and GSS larvae showed an identical expression of different lineage-specific genes. GM-CSF/CSF: granulocyte-monocyte colony-stimulating factor/colony-stimulating factor; SCF/KITLG: stem cell factor/KIT ligand; CXCL12/ SDF1α:CXCL12/stromal cell derived factor 1α; GSS: GM-CSF/CSF, SCF/KITLG, CXCL12/SDF1α−expressing transgenic zebrafish.