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Figure Caption
Figure 3—figure supplement 4.
Two-photon image of rsCaMPARI-mRuby3 in five dpf zebrafish brain without prior exposure to marking light. Excitation wavelength is 960 nm with detection wavelength ranges of 490–534 nm and 570–695 nm for rsCaMPARI and mRuby3, respectively. Note the general lack of mRuby3 fluorescence in nascent neurons in the periphery of brain structures, presumably due to its long maturation time. Scale bar is 100 μm.
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