Figure 5

Figure 5

(A–C) Analysis using Tg(her4.3:EGFP;SAGFF234A;UAS:mmCherry) allows her4.3:EGFP⁺ cells to be examined relative to the Cherry⁺ migrating NC cell population that colonises the gut during development. (A) At 54 hpf, no GFP⁺ cells (green) are present in the gut and none are detected within the population of migrating NC cells (red), although NC cell-derived HuC/D⁺ ENS neurons are present at this time (blue). Single channels shown in high magnification view of boxed region (n = 30). (B) At 60 hpf, small numbers of weakly GFP expressing cells (green, arrows) can be seen within the streams of NC cells colonising the gut (red). GFP⁺ cells are seen in proximity to HuC/D⁺ cells (blue). Note strongly GFP expressing cells can be detected, but these cells do not form part of the NC cell migratory streams (red) and are outside of the gut (grey arrowhead), and are likely to be melanocytes (n = 30). Single channels shown in high magnification view of boxed region. (C) At 4 dpf, an increased number of both strongly and weakly GFP expressing cells (green, arrows) are found within the stream of migratory NC cells (red). Single channels shown in high magnification view of boxed region (n = 20). (D) At 7 dpf Tg(her4.3:EGFP) larvae GFP expressing cells (green, arrowheads) are closely associated with, but distinct from, HuC/D⁺ positive neurons (red) (n = 45). Occasionally HuC/D is seen to overlap with cells expressing low levels of GFP (open arrowheads). All confocal images are max projections of short confocal stacks. Scale bars in merge panels: 50 µm.