|
Fig. 3 The zebrafish mci RNA is not abundantly expressed maternally, but is expressed in MCC precursors of the kidney tubules. (A) End-point PCR on cDNA samples from 1.5, 5, 14, 18 and 24 hpf embryos showing that maternal contribution of the mci transcript is minimal (1.5 hpf). actin-b1 (β-act) was used as the loading control. (B, C) mci transcript, as revealed by in situ hybridization, is present in a salt-and-pepper pattern (asterisks) in the kidney tubules of 24 hpf embryos (wild-type mib sibling panel in B, and the uninjected wild-type embryo panel in C), corresponding to MCC precursor cells. In N-deficient backgrounds (the mib mutant panel in B, and jagged2a MO injected embryo in panel in C), mci expression is observed in a much larger population of cells in the pronephric ducts. The images are representative of 2 independently performed experiments. Scale bars = 100 μm.
Reprinted from Developmental Biology, 465(2), Zhou, F., Rayamajhi, D., Ravi, V., Narasimhan, V., Chong, Y.L., Lu, H., Venkatesh, B., Roy, S., Conservation as well as divergence in Mcidas function underlies the differentiation of multiciliated cells in vertebrates, 168-177, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.